產品資料
產品[

CRL-2785 TC-1 小鼠肺上皮細胞

]資料
如果您對該產品感興趣的話,可以 sendmsg
產品名稱: CRL-2785 TC-1 小鼠肺上皮細胞
產品型號: TC-1 小鼠肺上皮細胞
產品廠商: 美國標準生物品收藏中心(ATCC)
產品文檔: 無相關文檔


簡單介紹

CRL-2785 TC-1 小鼠肺上皮細胞 ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|。細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和培養條件!


CRL-2785 TC-1 小鼠肺上皮細胞 的詳細介紹

CRL-2785 TC-1 小鼠肺上皮細胞
ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|。細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和*優培養條件!
 

ATCC® Number: CRL-2785? Price:
Designations: TC-1 [JHU-1]
Depositors: TC Wu
Biosafety Level: 2 [Cells contain HPV-16 and c-Ha-ras viral DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology: epithelial
CRL-2785 TC-1 小鼠肺上皮細胞
Source: Organ: lung
Strain: C57BL/6
Disease: tumor
Cell Type: human papillomavirus 16 (HPV-16) E6/E7 and c-Ha-Ras cotransformed
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Restrictions: Part of the Johns Hopkins Special Collection
Applications: The TC-1 lung metastasis model can be used to test the efficacy of various E6/E7-specific vaccines and immunotherapeutic strategies [PubMed: 9724092].
The tumor cell line, TC-1 [tissue culture number one], was derived from primary lung epithelial cells of C57BL/6 mice.
The cells are positive for the expression of HPV-16 E7 [PubMed: 8548765].
Tumorigenic: Yes                          CRL-2785 TC-1 小鼠肺上皮細胞
Comments: The tumor cell line, TC-1 [tissue culture number one], was derived from primary lung epithelial cells of C57BL/6 mice. The cells were immortalized with the amphotropic retrovirus vector LXSN16E6E7 and subsequently transformed with the pVEJB plasmid expressing the activated human c-Ha-ras oncogene [PubMed: 8548765]. The transformed cells were selected with G418 and Hygromycin B [PubMed: 8548765]. The cells are positive for the expression of HPV-16 E7 [PubMed: 8548765].The TC-1 lung metastasis model can be used to test the efficacy of various E6/E7-specific vaccines and immunotherapeutic strategies [PubMed: 9724092].
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate supplemented with 0.1 mM nonessential amino acids, 90%; fetal bovine serum, 10%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Subculture before confluence.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
    Cultures can be established between 1 X 10(5) and 2 X 10(5) viable cells/cm2.Maintain cultures at a cell concentration between 2 X 10(5) and 4 X 10(5) viable cells/cm2. Do not exceed 6 X 10(5) cells/cm2..
  6. Incubate cultures at 37°C.

Interval: A subcultivation ratio of 1:3 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 24 hours
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
source culture:ATCC JHU-1
References: 57430: Lin KY, et al. Treatment of established tumors with a novel vaccine that enhances major histocompatibility class II presentation of tumor antigen. Cancer Res. 56: 21-26, 1996. PubMed: 8548765
88870: Chen CH, et al. Antigen-specific immunotherapy for human papillomavirus 16 E7-expressing tumors grown in the liver. J. Hepatol. 33: 91-98, 2000. PubMed: 10905591
88871: Ji H, et al. Antigen-specific immunotherapy for murine lung metastatic tumors expressing human papillomavirus type 16 E7 oncoprotein. Int. J. Cancer 78: 41-45, 1998. PubMed: 9724092
CRL-2785 TC-1 小鼠肺上皮細胞
 
 

產品留言
標題
聯系人
聯系電話
內容
驗證碼
點擊換一張
注:1.可以使用快捷鍵Alt+S或Ctrl+Enter發送信息!
2.如有必要,請您留下您的詳細聯系方式!

聯系我們
產品搜索

細胞系,ATCC細胞,ATCC菌種,細胞系目錄,ATCC,鼠傷寒沙門氏菌,atcc細胞庫,人淋巴瘤細胞,人食管癌細胞,ATCC 19404,ATCC 25922,ATCC 19146,微生物菌種,細胞株,標準菌株,ATCC中國,atcc上海代理,CMCC 菌株,ATCC菌株,細胞目錄,質控菌株,腫瘤細胞株,乳腺癌細胞系,人肝癌細胞系

產品目錄

Copyright? 2003-2020  上海復祥生物科技有限公司版權所有            電話:021-35080510
傳真:021-35080520    地址:上海市虹口區赤峰路630號南雁大廈1901室    郵編: 滬ICP備10013034號  
  

滬公網安備 31010902002429號

数字基带信号码型有