產品資料
產品[

CRL-2647 L Wnt-3A 小鼠皮下結締組織細胞

]資料
如果您對該產品感興趣的話,可以 sendmsg
產品名稱: CRL-2647 L Wnt-3A 小鼠皮下結締組織細胞
產品型號: CRL-2647
產品廠商: 美國標準生物品收藏中心(ATCC)
產品文檔: 無相關文檔


簡單介紹

CRL-2647 L Wnt-3A 小鼠皮下結締組織細胞,原代細胞|細胞系|細胞株|菌種;細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和Z優培養條件!


CRL-2647 L Wnt-3A 小鼠皮下結締組織細胞 的詳細介紹
CRL-2647 L Wnt-3A 小鼠皮下結締組織細胞
ATCC® Number: CRL-2647?    Price: $289.00
Designations: L Wnt-3A
Depositors:  R Nusse
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus (mouse)
Morphology: fibroblast

Source: Tissue: subcutaneous connective tissue; areolar and adipose
Strain: C3H/An
Cellular Products: Wnt-3A protein
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Age: 100 days
Gender: male
Comments: L-M(TK-) cells (ATCC CCL-1.3) were transfected with a Wnt-3A expression vector and stable clones were selected in medium containing G418.
The Wnt-3A gene encodes a secreted glyoprotein with a variety of signaling effects. Wnt genes control many of the patterning and growth events during embryonic development.
The cells secrete biologically active Wnt-3A protein. They are presently the best source for production of Wnt-3A conditioned medium.
Since the conditioned medium contains other factors besides the Wnt-3A protein, it is necessary to control any experiments involving the Wnt-3A conditioned medium with control conditioned medium from the parental cell line (ATCC CRL-2648).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 0.4 mg/ml G-418; fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Protocol for Wnt-3A conditioned medium:
1. Split the cells 1:10 in 10 ml culture medium ( without G418 if conditioned medium is to be used with a cell line sensitive to G418) in 10 cm tissue culture dishes or T-75 flasks and let the cells grow for 4 days (approximately to confluency).2. Take off the medium and sterile filter. This is the first batch of medium.3. Add 10 ml fresh culture medium and culture for another 3 days.4. Take off the medium and sterile filter. This is the second batch of medium. Discard the cells, because they will be overgrown.5. Mix the first batch and second batch of medium 1:1. This is the Wnt-3A conditioned medium. It is stable at 4° C and can be frozen.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
parental cell line:ATCC CRL-2648
References: 90267: Willert K, et al. Wnt proteins are lipid-modified and can act as stem cell growth factors. Nature 423: 448-452, 2003. PubMed: 12717451
產品留言
標題
聯系人
聯系電話
內容
驗證碼
點擊換一張
注:1.可以使用快捷鍵Alt+S或Ctrl+Enter發送信息!
2.如有必要,請您留下您的詳細聯系方式!

聯系我們
產品搜索

細胞系,ATCC細胞,ATCC菌種,細胞系目錄,ATCC,鼠傷寒沙門氏菌,atcc細胞庫,人淋巴瘤細胞,人食管癌細胞,ATCC 19404,ATCC 25922,ATCC 19146,微生物菌種,細胞株,標準菌株,ATCC中國,atcc上海代理,CMCC 菌株,ATCC菌株,細胞目錄,質控菌株,腫瘤細胞株,乳腺癌細胞系,人肝癌細胞系

產品目錄

Copyright? 2003-2020  上海復祥生物科技有限公司版權所有            電話:021-35080510
傳真:021-35080520    地址:上海市虹口區赤峰路630號南雁大廈1901室    郵編: 滬ICP備10013034號  
  

滬公網安備 31010902002429號

数字基带信号码型有 上证指数走势图 浙江十一选五组选2奖金 安徽11选5走势图彩经网 体彩上海十一选五奖金 常山股份投资价值 喜乐彩票网官方网站 内蒙古十一选五开奖结果一定牛 股票指数期货交易策略及风险管理研究 福彩3d预测软件安卓版 2017年最赚钱的小生意 腾讯分分彩定位公式 河北十一选五软件下载 11选5开奖结果走势图 北京pk10预测 湖南快乐十分遗漏 真准网 中国平安股票行情东方财富