產品資料
產品[

CCL-226 C3H/10T1/2, Clone 8 小鼠胚胎成纖維細胞

]資料
如果您對該產品感興趣的話,可以 sendmsg
產品名稱: CCL-226 C3H/10T1/2, Clone 8 小鼠胚胎成纖維細胞
產品型號: CCL-226
產品廠商: 美國標準生物品收藏中心(ATCC)
產品文檔: 無相關文檔


簡單介紹

CCL-226 C3H/10T1/2, Clone 8 小鼠胚胎成纖維細胞,原代細胞|細胞系|細胞株|菌種;細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和*優培養條件!


CCL-226 C3H/10T1/2, Clone 8 小鼠胚胎成纖維細胞 的詳細介紹
CCL-226 C3H/10T1/2, Clone 8 小鼠胚胎成纖維細胞
ATCC® Number: CCL-226™    Price: $269.00
Designations: C3H/10T1/2, Clone 8
Depositors:  C Heidelberger
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties:adherent
Organism:Mus musculus (mouse)
Morphology:fibroblast
CCL-226 C3H/10T1/2, Clone 8 小鼠胚胎成纖維細胞
Source:Strain: C3H
Organ: embryo
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Applications:transfection host (Roche FuGENE® Transfection Reagents)
Tumorigenic:No
Reverse Transcript:negative
Antigen Expression:H-2k
Cytogenetic Analysis:Mouse karyotype with a modal number of 80 chromosomes.
Age: embryo
Comments:C3H/10T1/2, Clone 8 was isolated by C. Reznikoff, D. Brankow and C. Heidelberger in 1972 from a line of C3H mouse embryo cells. [23019]
The cells are very sensitive to post confluence inhibition of cell division, do not produce tumors in syngeneic mice, have no background of spontaneous transformation, nor do they contain overt endogenous transforming murine leukemia or sarcoma viruses. [22697]
The cells are contact sensitive.
There is no detectable background spontaneous transformation.
They are highly susceptible to transformation by chemical agents. [1208]
Tested and found negative for ectromelia virus (mousepox).
NOTE: THE INOCULATION DENSITY, FEEDING AND HARVESTING SCHEDULES MUST BE FOLLOWED RIGIDLY IF THE LINE IS TO RETAIN ITS ESSENTIAL CHARACTERISTICS.
THE BATCH OF SERUM USED FOR GROWTH AND FOR TRANSFORMATION ASSAYS MAY AFFECT BOTH THE MORPHOLOGY OF THIS LINE AND THE RESULTS OBTAINED.
Monolayers established and maintained for the standard transformation assay should be free of all foci after 6 weeks. [1208]
The donor recommends that the line be used between the 5th and 15th passages only.
Propagation: ATCC complete growth medium: The base medium for this cell line is Eagle's Basal medium with 2 mM L-glutamine , 1.5 g/L sodium bicarbonate and Earle's BSS. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
產品留言
標題
聯系人
聯系電話
內容
驗證碼
點擊換一張
注:1.可以使用快捷鍵Alt+S或Ctrl+Enter發送信息!
2.如有必要,請您留下您的詳細聯系方式!

聯系我們
產品搜索

細胞系,ATCC細胞,ATCC菌種,細胞系目錄,ATCC,鼠傷寒沙門氏菌,atcc細胞庫,人淋巴瘤細胞,人食管癌細胞,ATCC 19404,ATCC 25922,ATCC 19146,微生物菌種,細胞株,標準菌株,ATCC中國,atcc上海代理,CMCC 菌株,ATCC菌株,細胞目錄,質控菌株,腫瘤細胞株,乳腺癌細胞系,人肝癌細胞系

產品目錄

Copyright? 2003-2020  上海復祥生物科技有限公司版權所有            電話:021-35080510
傳真:021-35080520    地址:上海市虹口區赤峰路630號南雁大廈1901室    郵編: 滬ICP備10013034號  
  

滬公網安備 31010902002429號

数字基带信号码型有