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CRL-1714 TM3 正常小鼠睪丸Leydig細胞

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產品名稱: CRL-1714 TM3 正常小鼠睪丸Leydig細胞
產品型號: CRL-1714
產品廠商: 美國標準生物品收藏中心(ATCC)
產品文檔: 無相關文檔


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CRL-1714 TM3 正常小鼠睪丸Leydig細胞,原代細胞|細胞系|細胞株|菌種;細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和*優培養條件!


CRL-1714 TM3 正常小鼠睪丸Leydig細胞 的詳細介紹
CRL-1714 TM3 正常小鼠睪丸Leydig細胞
ATCC® Number: CRL-1714™    Price:
Designations: TM3
Depositors:  JP Mather
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus (mouse)
Morphology: epithelial

Source: Organ: testis
Disease: normal
Cell Type: Leydig cell;
Cellular Products: prostaglandin F2a
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Receptors: luteinizing hormone (LH); epidermal growth factor (EGF); androgen receptor, positive; estrogen receptor, positive; progesterone receptor, positive
Tumorigenic: No
Age: 11 to 13 days
Gender: male
Comments: The TM3 cell line responds to LH with an increase in cAMP production, but does not respond to follicle stimulating hormone (FSH).
The maintenance of responsiveness to LH is dependent upon serum lot.
In the presence of LH, the cells are capable of metabolizing cholesterol.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: A 1:1 mixture of Ham's F12 medium and Dulbecco's modified Eagle's medium with 2.5mM L-Glutamine, 0.5mM Sodium Pyruvate, 1.2 g/L Sodium Bicarbonate and 15 mM HEPES, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%
Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin-0.53mM EDTA solution, rinse, remove trypsin and let the culture sit at room temperature for 2 to 4 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:20 to 1:200 is recommended
Medium Renewal: Every 3 to 4 days
Preservation: Freeze medium: Complete growth medium, 95%, DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
References: 1158: Mather JP. Establishment and characterization of two distinct mouse testicular epithelial cell lines. Biol. Reprod. 23: 243-252, 1980. PubMed: 6774781
1159: Mather JP, et al. Culture of testicular cells in hormone-supplemented serum-free medium. Ann. N.Y. Acad. Sci. 383: 44-68, 1982. PubMed: 7046561
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