產品資料
產品[

CRL-2279 MS1 小鼠胰島內皮細胞

]資料
如果您對該產品感興趣的話,可以 sendmsg
產品名稱: CRL-2279 MS1 小鼠胰島內皮細胞
產品型號: CRL-2279
產品廠商: 美國標準生物品收藏中心(ATCC)
產品文檔: 無相關文檔


簡單介紹

CRL-2279 MS1 小鼠胰島內皮細胞,原代細胞|細胞系|細胞株|菌種;細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和*優培養條件!


CRL-2279 MS1 小鼠胰島內皮細胞 的詳細介紹

CRL-2279 MS1 小鼠胰島內皮細胞
ATCC® Number:  CRL-2279?       
Designations:  MS1 (MILE SVEN 1) 
Depositors:   JL Arbiser 
Biosafety Level: 2 [CELLS CONTAIN PAPOVAVIRUS ] 
Shipped:  frozen 
Medium & Serum:  See Propagation 
Growth Properties: adherent
Organism: Mus musculus (mouse) 
Morphology: endothelial

 
Source: Organ: pancreas
Strain: C57BL/6
Tissue: islet of Langerhans; endothelium
Cell Type: SV40 transformed
Cellular Products: tissue inhibitor of bioreactive matrix metalloproteinase (high levels) 
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 
 
Receptors: vascular endothelial growth factor (VEGF), expressed
Comments: MS1 is a pancreatic islet endothelial cell line established in 1994.
Primary islet endothelial cells were transduced with a temperature sensitive SV40 large T antigen (tsA-58-3) constructed and screened for resistance to G418.
Resistant colonies were isolated in cloning rings and screened for uptake of diI-Ac-LDL.
The line retains many properties of endothelial cells including uptake of acetylated LDL and expression of both Factor VIII related antigen and VEGF receptor.
The cells are useful for the study of signal transduction of angigenic factors.
It is the only line known that can give rise to benign hemangiomas.
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing:  Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Preservation:  Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 22603: Arbiser JL, et al. Oncogenic H-ras stimulates tumor angiogenesis by two distinct pathways. Proc. Natl. Acad. Sci. USA 94: 861-866, 1997. PubMed: 9023347
51452: Arbiser JL, et al. Overexpression of VEGF 121 in immortalized endothelial cells causes conversion to slowly growing angiosarcoma and high level expression of the VEGF receptors VEGFR-1 and VEGFR-2 in vivo. Am. J. Pathol. 156: 1469-1476, 2000. PubMed: 10751370 
 
 

產品留言
標題
聯系人
聯系電話
內容
驗證碼
點擊換一張
注:1.可以使用快捷鍵Alt+S或Ctrl+Enter發送信息!
2.如有必要,請您留下您的詳細聯系方式!

聯系我們
產品搜索

細胞系,ATCC細胞,ATCC菌種,細胞系目錄,ATCC,鼠傷寒沙門氏菌,atcc細胞庫,人淋巴瘤細胞,人食管癌細胞,ATCC 19404,ATCC 25922,ATCC 19146,微生物菌種,細胞株,標準菌株,ATCC中國,atcc上海代理,CMCC 菌株,ATCC菌株,細胞目錄,質控菌株,腫瘤細胞株,乳腺癌細胞系,人肝癌細胞系

產品目錄

Copyright? 2003-2020  上海復祥生物科技有限公司版權所有            電話:021-35080510
傳真:021-35080520    地址:上海市虹口區赤峰路630號南雁大廈1901室    郵編: 滬ICP備10013034號  
  

滬公網安備 31010902002429號

数字基带信号码型有 二分时时彩开奖官网 北京快乐8开奖app 爱彩乐内蒙古十一选五 安徽省十一选五一定牛 福建快3一定牛遗漏 新疆体彩十一选五 江西多乐彩投注技巧 广东十一选五下午精选 11选5任五210注万能码 北京单场快中彩 甘肃快三走形态走势图 如何购买股票操作流程 双色球今日历史同期号 极速赛车开奖网 浙江十一选五开奖历史 上海有哪些期货配资